Article de revue
Portable, on-demand biomolecular manufacturing
Here, we present a series of vignettes describing the production of a diverse set of therapeutics and molecular tools for clinical and research environments using freeze-dried, cell-free (FD-CF) reaction pellets. Nested within this proof of concept is a drive to create inexpensive alternatives for developing world applications where cost is a major factor to access. We begin with the production of two therapeutic classes of molecules: antimicrobial peptides (AMPs) and vaccines. For the former, we demonstrate purification schemes and validate antimicrobial activity. For the latter, we verify the expression of three vaccine antigens, including the scaled-up production and functional characterization of the diphtheria toxoid antigen (DT), which is administered to an animal model to confirm a successful immune response. Next, we establish a novel combinatorial approach to generate 90 possible affinity conjugates for applications in research and healthcare, of which a subset are functionally validated. Finally, we reconstitute a multi-enzyme biosynthesis pathway for small-molecular therapeutic production and offer a mix-and-match method to synthesize multiple pathway products, which are confirmed using mass spectrometry (MS).
Auteurs
Langues
- Anglais
Année de publication
2016
Journal
Cell
Volume
1
Type
Article de revue
Catégories
- Vaccins et dispositifs d'administration
Maladies
- Diphtérie
Mots-clés
- Demande
- Introduction de nouveaux vaccins
