Thanks Julian. It really helped. I stress so much on anti freezing the H1N1 vaccine during my country wide lectures that I needed to be sure of my claims. Frankly it would have been better for a H1N1 vccine without VVM to be freeze resistant, as opertaionally it is much easier for vaccinators in countries like ours to keep a vaccine close to ice and hence close to 0 or below than to keep it close to +2-+8C. Thanks again Best regards