Guidance

Annex C17 Generating Consensus Sequences with Sequencher

Purpose
The purpose of the procedure is to derive the sequence of the 450 nucleotides encoding the carboxyl terminus of the N gene of measles virus, or the 739 nucleotides encoding the sequencing window of the E1 gene of rubella virus, or the approximately 1018 nucleotides from the non-coding region of the measles virus between the M and F coding regions (MF-NCR), from an existing PCR product.

Authors

Languages

  • English

Publication year

2024

Type

Guidance

Categories

  • Service delivery

Topic references

GROUP-C

TitleAuthorYearTypeLanguage
Annex C01. Measles genotyping RT-PCR (CDC)Mick N. Mulders2020GuidanceEnglish
Annex C01a. Worksheet for Annex C1, C2, C3.Mick N. Mulders2020GuidanceEnglish
Annex C02. Protocol for rubella genotyping RT-PCR, Qiagen (CDC)Mick N. Mulders2020GuidanceEnglish
Annex C03. Protocol for rubella genotyping RT-PCR, Invitrogen Superscript III (CDC)Mick N. Mulders2020GuidanceEnglish
Annex C04. Measles sequencing protocol for genotyping (CDC)Mick N. Mulders2020GuidanceEnglish
Annex C04a. Measles sequencing worksheet for Annex C4Mick N. Mulders2020GuidanceEnglish
Annex C05. Rubella sequencing protocol for genotyping (CDC)Mick N. Mulders2020GuidanceEnglish
Annex C05a. Rubella sequencing worksheet for Annex C5Mick N. Mulders2020GuidanceEnglish
Annex C06. Measles sequence analysis for genotyping using MEGA v6.06Mick N. Mulders2020GuidanceEnglish
Annex C07. Rubella sequence analysis for genotyping using MEGA v5.05Mick N. Mulders2020GuidanceEnglish
Annex C08. Measles & rubella sequence assembly (editing) using GeneStudioMick N. Mulders2020GuidanceEnglish
Annex C09. Phylogenetic analysis (MEGA v7 or X) of measles and rubella sequences assembled using GenestudioMick N. Mulders2020GuidanceEnglish
Annex C10. Agarose gel electrophoresis with measles and rubella synthetic positive controlsMick N. Mulders2020GuidanceEnglish
Annex C13. Directions for finding and using the MeaNS distinct sequence IDMick N. Mulders2021GuidanceEnglish
Annex C14. Creating a Table and Chart to Describe Genotyping DataMick N. Mulders2021GuidanceEnglish
Annex C15. Using the RECall programMick N. Mulders2024User guideEnglish
Annex C16: CDC Protocol for amplification and sequencing of measles virus MF-NCR from patient samplesMick N. MuldersGuidanceEnglish
Annex C17 Generating Consensus Sequences with SequencherMick N. Mulders2024GuidanceEnglish

Added by: Mick Mulders

Added on: 2024-11-07 16:21:32

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