List of Annexes

Mick Mulders


Annexes to the Laboratory Manual Homepage

The annexes provide basic test method details or guidance documents to augment the material in the laboratory manual. The annex materials and protocols are not intended to provide laboratories with a standard operating protocol (SOP) as is, but may serve as a template for each individual laboratory to develop SOPs, and to stay current with new techniques as appropriate according to availability and resources.
The inclusion of reagents and products from specific manufacturers does not constitute an endorsement by the WHO or the GMRLN.

The documents are organized by subject matter into groups designated by a letter (A-F) and a number. Each annex in the list is assigned a consecutive number. If a document is superseded by a revised version, the new version will have a numeric extension. For example, if a revised version of an existing document named Annex B5 becomes available, the revised document will replace the original document and will be referred to as Annex B5.2.

New protocols will be added to the appropriate group with the next consecutive number at the end of the existing list.

The annex groups are organized according to subject matter described below. Click on the annex group below to navigate to the page with the directory (list) of the individual protocols (and links to pdfs) within the annex group.

TitleAuthorYearTypeLanguage
Annex A01. Measles and rubella laboratory request formMick N. Mulders2020GuidanceEnglish
Annex A02. Measles and rubella investigation formMick N. Mulders2020GuidanceEnglish
Annex A03. Shipping clinical specimensMick N. Mulders2020GuidanceEnglish
Annex A04. Dried blood spot (DBS) protocolsMick N. Mulders2020GuidanceEnglish
Annex A06. Preparation and use of FTA cardsMick N. Mulders2020GuidanceEnglish

TitleAuthorYearTypeLanguage
Annex B01. Comparison of measles and rubella IgM EIA kitsMick N. Mulders2020GuidanceEnglish
Annex B02. EuroImmun protocol for measles IgM ELISAMick N. Mulders2020GuidanceEnglish
Annex B02a. Job aid for Annex B2 (and B3)Mick N. Mulders2020GuidanceEnglish
Annex B02b. EuroImmun IgM ELISA Manual Calculation WorksheetMick N. Mulders2020GuidanceEnglish
Annex B02c. EuroImmun IgM ELISA Worksheet with built-in calculationsMick N. Mulders2020GuidanceEnglish
Annex B03. EuroImmun protocol for rubella IgM ELISAMick N. Mulders2020GuidanceEnglish
Annex B04. Serion protocol for measles IgM ELISAMick N. Mulders2020GuidanceEnglish
Annex B04a. Job Aid for Serion ELISA Classic Measles-rubella Virus IgMMick N. Mulders2020GuidanceEnglish
Annex B04b. Serion IgM ELISA Manual Calculation WorksheetMick N. Mulders2020GuidanceEnglish
Annex B04c. Serion IgM ELISA Worksheet with built-in calculationsMick N. Mulders2020GuidanceEnglish
Annex B05. Serion protocol for rubella IgM ELISAMick N. Mulders2020GuidanceEnglish
Annex B07. Propagation and maintenance of Vero-hSLAM cell culture (CDC)Mick N. Mulders2020GuidanceEnglish
Annex B08. Measles or rubella virus isolation and preparation of virus stocksMick N. Mulders2021GuidanceEnglish
Annex B09. Confirmation of rubella virus in culture by IFA and ICAMick N. Mulders2020GuidanceEnglish
Annex B10. RNA extraction methods for clinical samples and FTA cardsMick N. Mulders2020GuidanceEnglish
Annex B11. Measles real-time RT-PCR, N gene (CDC)Mick N. Mulders2020GuidanceEnglish
Annex B11.2 Real-time RT-PCR Assays for the Detection of Measles Virus (MeV) N Gene RNA and Human RNase P mRNA (a cellular reference gene) using the ABI 7500 Real-Time ThermocyclerMick N. Mulders2020GuidanceEnglish
Annex B11.2A. Invitrogen Real Time PCR TemplateMick N. Mulders2020GuidanceEnglish
Annex B11a. Worksheet (duplicate wells) for Annex B11Mick N. Mulders2020GuidanceEnglish
Annex B11b. Plate layout (duplicate wells) for Annex B11Mick N. Mulders2020GuidanceEnglish
Annex B11c. Worksheet (triplicate wells) for Annex B11Mick N. Mulders2020GuidanceEnglish
Annex B12. Rubella real-time RT-PCR (NSP)Mick N. Mulders2020GuidanceEnglish
Annex B12.2. Real-time RT-PCR Assays for the Detection of Rubella Virus RNA and Human RNase P mRNA using the ABI 7500 Real-time ThermocyclerMick N. Mulders2021GuidanceEnglish
Annex B12.2a. Invitrogen Real Time PCR Template: RubellaMick N. Mulders2020GuidanceEnglish
Annex B12.2b. Invitrogen Real Time PCR Template: Rubella with Diluted ROXMick N. Mulders2020GuidanceEnglish
Annex B12a. Worksheet for Annex B12Mick N. Mulders2020GuidanceEnglish
Annex B12b. RuV Real-Time Layout (ROX dilution)Mick N. Mulders2020GuidanceEnglish
Annex B12c. RuV Real-Time LayoutMick N. Mulders2020GuidanceEnglish
Annex B13. Clean-up of PCR ProductsMick N. Mulders2021GuidanceEnglish

TitleAuthorYearTypeLanguage
Annex C01. Measles genotyping RT-PCR (CDC)Mick N. Mulders2020GuidanceEnglish
Annex C01a. Worksheet for Annex C1, C2, C3.Mick N. Mulders2020GuidanceEnglish
Annex C02. Protocol for rubella genotyping RT-PCR, Qiagen (CDC)Mick N. Mulders2020GuidanceEnglish
Annex C03. Protocol for rubella genotyping RT-PCR, Invitrogen Superscript III (CDC)Mick N. Mulders2020GuidanceEnglish
Annex C04. Measles sequencing protocol for genotyping (CDC)Mick N. Mulders2020GuidanceEnglish
Annex C04a. Measles sequencing worksheet for Annex C4Mick N. Mulders2020GuidanceEnglish
Annex C05. Rubella sequencing protocol for genotyping (CDC)Mick N. Mulders2020GuidanceEnglish
Annex C05a. Rubella sequencing worksheet for Annex C5Mick N. Mulders2020GuidanceEnglish
Annex C06. Measles sequence analysis for genotyping using MEGA v6.06Mick N. Mulders2020GuidanceEnglish
Annex C07. Rubella sequence analysis for genotyping using MEGA v5.05Mick N. Mulders2020GuidanceEnglish
Annex C08. Measles & rubella sequence assembly (editing) using GeneStudioMick N. Mulders2020GuidanceEnglish
Annex C09. Phylogenetic analysis (MEGA v7 or X) of measles and rubella sequences assembled using GenestudioMick N. Mulders2020GuidanceEnglish
Annex C10. Agarose gel electrophoresis with measles and rubella synthetic positive controlsMick N. Mulders2020GuidanceEnglish
Annex C13. Directions for finding and using the MeaNS distinct sequence IDMick N. Mulders2021GuidanceEnglish
Annex C14. Creating a Table and Chart to Describe Genotyping DataMick N. Mulders2021GuidanceEnglish
Annex C15. Using the RECall programMick N. Mulders2022User guideEnglish
Annex C16: CDC Protocol for amplification and sequencing of measles virus MF-NCR from patient samplesMick N. MuldersGuidanceEnglish
Annex C17. Generating Consensus Sequences with SequencherMick N. MuldersGuidanceEnglish

TitleAuthorYearTypeLanguage
Annex D01. Levey–Jennings control chartsMick N. Mulders2020GuidanceEnglish
Annex D02. Supplemental information for optimal laboratory designMick N. Mulders2020GuidanceEnglish
Annex D05. Verification for new IgM EIAsMick N. Mulders2021GuidanceEnglish

Resources not available.

 

For inquiries, please contact:
Dr. Mick N. Mulders, Scientist
Coordinator Global VPD Laboratory Networks
Expanded Programme on Immunization (EPI)
Department of Immunization, Vaccines & Biologicals (IVB)
Universal Health Coverage/Lifecourse Division (UHC/LC)
Mail: This email address is being protected from spambots. You need JavaScript enabled to view it.