Guidance
Annex A07. Evaluating the Use of RT-qPCR Alone for Discarding Measles Cases: A Review of Current Evidence and Global Practices
This paper evaluates the feasibility of using RT-qPCR as the sole diagnostic method for discarding suspected measles cases, replacing traditional IgM serology. Drawing on global data, laboratory practices, and diagnostic performance metrics, we assess the sensitivity, specificity, and operational implications of a PCR-only approach. We conclude with a proposal for updated diagnostic criteria and highlight the need for further data to support policy changes.
Authors
Languages
- English
Publication year
2025
Type
Guidance
Categories
- Programme management
Diseases
- Measles
GROUP-A
| Title | Author | Year | Type | Language |
|---|---|---|---|---|
| Annex A04. Dried blood spot (DBS) protocols | Mick N. Mulders | 2020 | Guidance | English |
| Annex A06. Preparation and use of FTA cards | Mick N. Mulders | 2020 | Guidance | English |
| Annex A03. Shipping clinical specimens | Mick N. Mulders | 2020 | Guidance | English |
| Annex A02. Measles and rubella investigation form | Mick N. Mulders | 2020 | Guidance | English |
| Annex A01. Measles and rubella laboratory request form | Mick N. Mulders | 2020 | Guidance | English |
| Annex A07. Evaluating the Use of RT-qPCR Alone for Discarding Measles Cases: A Review of Current Evidence and Global Practices | Kevin E. Brown | 2025 | Guidance | English |
| Annex A08. Global Measles Rubella Laboratory Network (GMRLN): Guidance for Laboratories during Measles Outbreaks | Paul A. Rota | 2025 | Guidance | English |
