Guidance

Annex B13. Clean-up of PCR Products

Mick N. Mulders

The following protocol provides recommendations for the purification of DNA fragments from positive PCR reactions.

Authors

Languages

  • English

Publication year

2021

Type

Guidance

Categories

  • Data

Diseases

  • Measles

Organisations

  • World Health Organization (WHO)

GROUP-B

The resource is included in the following table:

TitleAuthorYearTypeLanguage
Annex B01. Comparison of measles and rubella IgM EIA kitsMick N. Mulders2020GuidanceEnglish
Annex B12.2a. Invitrogen Real Time PCR Template: RubellaMick N. Mulders2020GuidanceEnglish
Annex B11.2A. Invitrogen Real Time PCR TemplateMick N. Mulders2020GuidanceEnglish
Annex B12.2b. Invitrogen Real Time PCR Template: Rubella with Diluted ROXMick N. Mulders2020GuidanceEnglish
Annex B11.2 Real-time RT-PCR Assays for the Detection of Measles Virus (MeV) N Gene RNA and Human RNase P mRNA (a cellular reference gene) using the ABI 7500 Real-Time ThermocyclerMick N. Mulders2020GuidanceEnglish
Annex B12.2. Real-time RT-PCR Assays for the Detection of Rubella Virus RNA and Human RNase P mRNA using the ABI 7500 Real-time ThermocyclerMick N. Mulders2021GuidanceEnglish
Annex B08. Measles or rubella virus isolation and preparation of virus stocksMick N. Mulders2021GuidanceEnglish
Annex B13. Clean-up of PCR ProductsMick N. Mulders2021GuidanceEnglish
Annex B04a. Job Aid for Serion ELISA Classic Measles-rubella Virus IgMMick N. Mulders2020GuidanceEnglish
Annex B04. Serion protocol for measles IgM ELISAMick N. Mulders2020GuidanceEnglish
Annex B03. EuroImmun protocol for rubella IgM ELISAMick N. Mulders2020GuidanceEnglish
Annex B02c. EuroImmun IgM ELISA Worksheet with built-in calculationsMick N. Mulders2020GuidanceEnglish
Annex B02b. EuroImmun IgM ELISA Manual Calculation WorksheetMick N. Mulders2020GuidanceEnglish
Annex B02a. Job aid for Annex B2 (and B3)Mick N. Mulders2020GuidanceEnglish
Annex B02. EuroImmun protocol for measles IgM ELISAMick N. Mulders2020GuidanceEnglish
Annex B12c. RuV Real-Time LayoutMick N. Mulders2020GuidanceEnglish
Annex B12b. RuV Real-Time Layout (ROX dilution)Mick N. Mulders2020GuidanceEnglish
Annex B12a. Worksheet for Annex B12Mick N. Mulders2020GuidanceEnglish
Annex B12. Rubella real-time RT-PCR (NSP)Mick N. Mulders2020GuidanceEnglish
Annex B11c. Worksheet (triplicate wells) for Annex B11Mick N. Mulders2020GuidanceEnglish
Annex B11b. Plate layout (duplicate wells) for Annex B11Mick N. Mulders2020GuidanceEnglish
Annex B11a. Worksheet (duplicate wells) for Annex B11Mick N. Mulders2020GuidanceEnglish
Annex B11. Measles real-time RT-PCR, N gene (CDC)Mick N. Mulders2020GuidanceEnglish
Annex B10. RNA extraction methods for clinical samples and FTA cardsMick N. Mulders2020GuidanceEnglish
Annex B09. Confirmation of rubella virus in culture by IFA and ICAMick N. Mulders2020GuidanceEnglish
Annex B07. Propagation and maintenance of Vero-hSLAM cell culture (CDC)Mick N. Mulders2020GuidanceEnglish
Annex B05. Serion protocol for rubella IgM ELISAMick N. Mulders2020GuidanceEnglish
Annex B04c. Serion IgM ELISA Worksheet with built-in calculationsMick N. Mulders2020GuidanceEnglish
Annex B04b. Serion IgM ELISA Manual Calculation WorksheetMick N. Mulders2020GuidanceEnglish

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