This guide supports targeted efforts to operationalise Gavi, the Vaccine Alliance’s Digital Health Information Strategy which includes “gender-intentional” in the vision and prioritisation of gender-related activities that can support scale and impact by overcoming negative gender biases, gender dynamics, and the effects of the gender digital divide on digital health interventions. During the development of National Country Roadmaps for Digital Health Information (DHI) for Immunisation as part of the Gavi DHI Strategy testing process, it was clear that while gender was included as a priority in the National Immunisation Strategy, it was unclear which aspects of gender or how it would be addressed. In addition, it was difficult to engage in meaningful conversations related to gender among immunisation and digital health stakeholders during sub-national and national-level workshops. As time and resources are limited it was advised that any gender-related resource would need to be quick and easy to use with the aim of helping countries, health systems, and their digital health interventions move incrementally across a continuum of gender programming towards gender transformation, programme development and design that promotes equality, diversity and inclusion for all users.